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	Provedor de dados BJPS (1) Braz. J. Vet. Res. Anim. Sci. (1) Autor BACILA,Metry (1) Deters,Alexandra (1) Hensel,Andreas (1) Löhr,Gesine (1) MALUCELLI,Maria Ivette Carboni (1) RICHARTZ,Rosária Regina Tesoni B. (1) SALVO,Ligia Maria (1) Mais... Palavra-chave Liver cells (2) Artichoke (1) Cell culture (1) Cell proliferation (1) Cells (1) Cynara scolymus L. Asteraceae (1) Gene expression (1) Metynnis roosevelti (1) Pisces (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2009 (1) 2000 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última In vitro investigations of Cynara scolymus L. extract on cell physiology of HepG2 liver cells BJPS Löhr,Gesine; Deters,Alexandra; Hensel,Andreas. The objective of this study was the investigation of a potential influence of artichoke leaf extract (ALE) on the cell physiology and gene expression of phase I/II enzymes of human liver cells HepG2 and investigation on potential cell protective effects against ethanol-induced cell toxicity against HepG2 cells. Cell biological assays under in vitro conditions using HepG2 liver cells and investigation of mitochondrial activity (MTT test), proliferation assay (BrdU incorporation ELISA), LDH as toxicity marker, gene expression analysis by RT-PCR and enzyme activity of glutationtransferase. Artichocke extract, containing 27% caffeoylquinic acids and 7% flavonoids induced mitochondrial activity, proliferation and total protein content under in vitro conditions... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cynara scolymus L. Asteraceae; Artichoke; Liver cells; Cell proliferation; Gene expression. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200003 Primary culture of hepatic cells from Metynnis roosevelti (Pisces, Teleostei, Characidae) Braz. J. Vet. Res. Anim. Sci. SALVO,Ligia Maria; MALUCELLI,Maria Ivette Carboni; RICHARTZ,Rosária Regina Tesoni B.; BACILA,Metry. In order to achieve monolayers and adhesion in cell cultures of hepatocytes from the subtropical fish Metynnis roosevelti (Pisces, Teleostei, Characidae) three different methodologies were used: enzyme cell dissociation by means of a system containing trypsin and versene 0.25%; by means of bovine fetal serum and by the use of blood serum obtained from adult specimens of M. roosevelti. The cell medium used has been a modified F10-199 by the addition of 2 mM L-glutamine, 10 µg/mL insulin, 50 µg/mL fibronectin; and antibiotics. The best results in terms of cell dissociation as well as in terms of adhesion and confluence of the cells monolayer has been achieved by means of the enzymatic cell dissociation. Tipo: Info:eu-repo/semantics/article Palavras-chave: Liver cells; Cell culture; Cells; Pisces; Metynnis roosevelti. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000500004 Registros recuperados: 2 Primeira ... 1 ... Última

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